Asparaginase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49617

Novus Biologicals, part of Bio-Techne
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NBP2-49617
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Asparaginase Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: VLVPGTGLAAILRTLPMFHDEEHARARGLSEDTLVLPPASRNQRILYTVLECQPLFDSSDMTIAEWVCLAQTIKRHYEQYHGFVVIHGTD

Reactivity Notes

Mouse (80%), Rat (80%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Asparaginase Antibody

Immunohistochemistry-Paraffin: Asparaginase Antibody [NBP2-49617]

Immunohistochemistry-Paraffin: Asparaginase Antibody [NBP2-49617]

Immunohistochemistry-Paraffin: Asparaginase Antibody [NBP2-49617] - Staining in human kidney and pancreas tissues using anti-ASPG antibody. Corresponding ASPG RNA-seq data are presented for the same tissues.
Western Blot: Asparaginase Antibody [NBP2-49617]

Western Blot: Asparaginase Antibody [NBP2-49617]

Western Blot: Asparaginase Antibody [NBP2-49617] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251 MG
Lane 4: Human plasma
Lane 5: Human Liver tissue
Immunohistochemistry-Paraffin: Asparaginase Antibody [NBP2-49617]

Immunohistochemistry-Paraffin: Asparaginase Antibody [NBP2-49617]

Immunohistochemistry-Paraffin: Asparaginase Antibody [NBP2-49617] - Staining of human kidney shows high expression.
View 1 more images

Applications for Asparaginase Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Asparaginase

Asparaginase is an enzyme purified from E. coli and Erwinia carotovora. It acts by deaminating extracellular L asparagine, an amino acid that appears to be essential for protein synthesis by some tumour cells which are unable to synthesise asparagine. Asparaginase from Erwinia carotovora is serologically and biochemically distinct from asparaginase from E. coli, although its antineoplastic activity and toxicity is similar. Asparaginase is usually considered to be cell cycle phase nonspecific, but it may block some cells in G1 or S phase. Asparaginase reduces cellular and humoral immunity. E.coli contains two L asparaginase isoenzymes: L asparaginase I, a low affinity enzyme located in the cytoplasm, and L asparaginase II, a high affinity secreted enzyme.

Long Name

Asparaginase

Alternate Names

ASPG, C14orf76

Gene Symbol

ASPG

Additional Asparaginase Products

Product Documents for Asparaginase Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Asparaginase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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