Asparagine synthetase Antibody (OTI1A10) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70215

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1A10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Asparagine Synthetase/ASNS Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ASNS(NP_597680) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

64.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Asparagine synthetase Antibody (OTI1A10) - Azide and BSA Free

Western Blot: Asparagine synthetase Antibody (OTI1A10)Azide and BSA Free [NBP2-70215]

Western Blot: Asparagine synthetase Antibody (OTI1A10)Azide and BSA Free [NBP2-70215]

Western Blot: Asparagine synthetase Antibody (OTI1A10) - Azide and BSA Free [NBP2-70215] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Asparagine synthetase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti
Immunocytochemistry/ Immunofluorescence: Asparagine synthetase Antibody (OTI1A10) - Azide and BSA Free [NBP2-70215]

Immunocytochemistry/ Immunofluorescence: Asparagine synthetase Antibody (OTI1A10) - Azide and BSA Free [NBP2-70215]

Immunocytochemistry/Immunofluorescence: Asparagine synthetase Antibody (OTI1A10) - Azide and BSA Free [NBP2-70215] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Asparagine synthetase.

Applications for Asparagine synthetase Antibody (OTI1A10) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Asparagine Synthetase/ASNS

Asparagine synthetase (AS) is a housekeeping enzyme responsible for the production of asparagine from aspartate and glutamine. Most mammalian cells express AS and regulate the level of activity in response to the concentration of asparagine in the medium. Certain tumor cells in contrast, exhibit little or no AS activity and rely on the surrounding medium as a source of exogenous asparagines (1). Therefore tumor cells may be selectively killed by a chemotherapeutic drug using asparaginase. This approach has been exploited in the treatment of certain cancers like childhood acute lymphoblastic leukemia (2).

Long Name

ASNS

Alternate Names

ASNSD, EC 6.3.5.4, Glutamine Dependent Asparagine Synthetase, TS11 Cell Cycle Control Protein

Gene Symbol

ASNS

Additional Asparagine Synthetase/ASNS Products

Product Documents for Asparagine synthetase Antibody (OTI1A10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Asparagine synthetase Antibody (OTI1A10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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