Aspartate Aminotransferase Antibody (3G11) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59483

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3G11

Format

BSA Free

Concentration

1 mg/ml

View all Aspartate Aminotransferase Antibodies »

Product Specifications

Immunogen

Recombinant human Aspartate Aminotransferase (1-413aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Aspartate Aminotransferase Antibody (3G11) - BSA Free

Western Blot: Aspartate Aminotransferase Antibody (3G11)BSA Free [NBP2-59483]

Western Blot: Aspartate Aminotransferase Antibody (3G11)BSA Free [NBP2-59483]

Western Blot: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - The recombinat proteins (100ng) were resolved by SDS PAGE, transferred to PVDF membrane and probed with antihuman GOT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : Recombinant human GOT1 Lane 2 : Recombinant human GOT2
Immunocytochemistry/ Immunofluorescence: Aspartate Aminotransferase Antibody (3G11) - BSA Free [NBP2-59483]

Immunocytochemistry/ Immunofluorescence: Aspartate Aminotransferase Antibody (3G11) - BSA Free [NBP2-59483]

Immunocytochemistry/Immunofluorescence: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - Analysis of GOT1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GOT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of GOT1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GOT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Flow Cytometry: Aspartate Aminotransferase Antibody (3G11) - BSA Free [NBP2-59483]

Flow Cytometry: Aspartate Aminotransferase Antibody (3G11) - BSA Free [NBP2-59483]

Flow Cytometry: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - Flow cytometry analysis of GOT1 in Hep3B cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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Applications for Aspartate Aminotransferase Antibody (3G11) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1080

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aspartate Aminotransferase

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]

Alternate Names

aspartate aminotransferase, cytoplasmic, ASTQTL1, CASPAT, CCAT, Cysteine aminotransferase, cytoplasmic, Cysteine transaminase, cytoplasmic, EC 2.6.1.1, EC 2.6.1.3, GIG18, Glutamate oxaloacetate transaminase 1, glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1), GOT1, growth-inhibiting protein 18, Transaminase A

Gene Symbol

GOT1

Additional Aspartate Aminotransferase Products

Product Documents for Aspartate Aminotransferase Antibody (3G11) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Aspartate Aminotransferase Antibody (3G11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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