Aspartate Aminotransferase Antibody (A2D6-R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32050
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # A2D6-R
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within Human Aspartate Aminotransferase aa 16-211 / 413. (Uniprot: P17174)
Localization
Cytoplasm.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Aspartate Aminotransferase Antibody (A2D6-R)
Western Blot: Aspartate Aminotransferase Antibody (A2D6-R) [NBP3-32050]
Western Blot: Aspartate Aminotransferase Antibody (A2D6-R) [NBP3-32050] - Western blot analysis of Aspartate Aminotransferase on different lysates with Mouse anti-Aspartate Aminotransferase antibody (NBP3-32050) at 1/1,000 dilution.Lane 1: HeLa cell lysate (20 ug/Lane)
Lane 2: K-562 cell lysate (20 ug/Lane)
Lane 3: HL-60 cell lysate (20 ug/Lane)
Lane 4: Caco-2 cell lysate (20 ug/Lane)
Lane 5: Human liver tissue lysate (40 ug/Lane)
Lane 6: Mouse liver tissue lysate (40 ug/Lane)
Lane 7: Mouse heart tissue lysate (40 ug/Lane)
Lane 8: Rat liver tissue lysate (40 ug/Lane)
Lane 9: Rat heart tissue lysate (40 ug/Lane)
Lysates/proteins at 10 ug/Lane.
Predicted band size: 46 kDa
Observed band size: 38 kDa
Exposure time: 1 minute 41 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32050) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for Aspartate Aminotransferase Antibody (A2D6-R)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Aspartate Aminotransferase
Alternate Names
aspartate aminotransferase, cytoplasmic, ASTQTL1, CASPAT, CCAT, Cysteine aminotransferase, cytoplasmic, Cysteine transaminase, cytoplasmic, EC 2.6.1.1, EC 2.6.1.3, GIG18, Glutamate oxaloacetate transaminase 1, glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1), GOT1, growth-inhibiting protein 18, Transaminase A
Gene Symbol
GOT1
Additional Aspartate Aminotransferase Products
Product Documents for Aspartate Aminotransferase Antibody (A2D6-R)
Product Specific Notices for Aspartate Aminotransferase Antibody (A2D6-R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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