Aspartate Aminotransferase Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-93403
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-RSYRYWDAEKR corresponding to internal region according to NP_002070.1.
Epitope
C-RSYRYWDAEKR
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Aspartate Aminotransferase Antibody
![Western Blot: Aspartate Aminotransferase Antibody [NB100-93403]](https://resources.bio-techne.com/images/products/Aspartate-Aminotransferase-Antibody-Western-Blot-NB100-93403-img0003.jpg "Western Blot: Aspartate Aminotransferase Antibody [NB100-93403]")
Western Blot: Aspartate Aminotransferase Antibody [NB100-93403]
Western Blot: Aspartate Aminotransferase Antibody [NB100-93403] - Staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.![Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NB100-93403]](https://resources.bio-techne.com/images/products/Aspartate-Aminotransferase-Antibody-Immunohistochemistry-Paraffin-NB100-93403-img0002.jpg "Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NB100-93403]")
Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NB100-93403]
Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NB100-93403] - Staining of human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.![Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NB100-93403]](https://resources.bio-techne.com/images/products/Aspartate-Aminotransferase-Antibody-Immunohistochemistry-Paraffin-NB100-93403-img0001.jpg "Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NB100-93403]")
Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NB100-93403]
Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NB100-93403] - Staining of human heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for Aspartate Aminotransferase Antibody
Application
Recommended Usage
Immunohistochemistry
2 - 5 ug/ml
Immunohistochemistry-Paraffin
2 - 5 ug/ml
Peptide ELISA
Detection limit 1:4000
Western Blot
0.01 - 1 ug/ml
Application Notes
WB: Approx 40 kDa band observed in human brain (cerebellum), Liver and skeletal muscle lysates (calculated MW of 46.2 kDa according to NP_002070.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (PMID: 2185471). IHC: Human heart shows strong staining of the capillary network between the myocardial fibres in transverse secion.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Aspartate Aminotransferase
Alternate Names
aspartate aminotransferase, cytoplasmic, ASTQTL1, CASPAT, CCAT, Cysteine aminotransferase, cytoplasmic, Cysteine transaminase, cytoplasmic, EC 2.6.1.1, EC 2.6.1.3, GIG18, Glutamate oxaloacetate transaminase 1, glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1), GOT1, growth-inhibiting protein 18, Transaminase A
Gene Symbol
GOT1
UniProt
Additional Aspartate Aminotransferase Products
Product Documents for Aspartate Aminotransferase Antibody
Product Specific Notices for Aspartate Aminotransferase Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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