Astrin Antibody

Immunocytochemistry/ Immunofluorescence: Astrin Antibody [NB100-74638]

Immunocytochemistry/Immunofluorescence: Astrin Antibody [NB100-74638] - Immunofluorescence images of control or CENP-E siRNA-transfected cells treated with monastrol for 1 hr prior to fixation and immunostained with antibodies against Astrin and tubulin and CREST antisera and stained with DAPI for DNA. Cropped images are 3x magnified showing lateral kinetochores. Scale bars represent 5 um (insets, 2 um). Image collected and cropped by CiteAb from the following publication (https://linkinghub.elsevier.com/retrieve/pii/S0960982213007653), licensed under a CC-BY license.

Immunoprecipitation: Astrin Antibody [NB100-74638]

Immunoprecipitation: Astrin Antibody [NB100-74638] - Detection of Human Astrin/MAP126 by Western Blot of Immunoprecipitants. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Astrin/MAP126 antibody NB100-74638 used for IP at 3 mcg/mg lysate. Astrin/MAP126 was also immunoprecipitated by another rabbit anti-Astrin/MAP126 antibody and NB100-74639, which recognize downstream epitopes. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry: Astrin Antibody [NB100-74638] -

Immunohistochemistry: Astrin Antibody [NB100-74638] - SKA3 localisation is not abrogated due to the lack of Astrin.(A) Representative deconvolved images show SKA3 enrichment at kinetochores of HeLa cells treated with Control or Astrin siRNA. Cells were arrested in metaphase using MG132 for 1 hr prior to fixation & were immunostained with antibodies against Astrin & SKA3 & CREST antiserum (to mark centromeres) & stained with DAPI for DNA. Cropped images show Astrin or SKA3 enrichment at kinetochores. (B) Representative images show SKA3 enrichment at kinetochores of HeLa cells treated with Astrin siRNA before transfection with eukaryotic protein expression vectors encoding YFP-Astrin (WT, 4A or ∆70, as indicated). Cells were arrested in metaphase using MG132 for 1 hr prior to fixation & were immunostained with antibodies against GFP (for detecting YFP-Astrin) & SKA3 & CREST antiserum & co-stained with DAPI for DNA. Cropped images show YFP-Astrin or SKA3 enrichment at kinetochores. Scale as indicated. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31808746), licensed under a CC-BY license. Not internally tested by Novus Biologicals.