ATAD2 Antibody (CL0182)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14786

Novus Biologicals, part of Bio-Techne
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NBP2-14786
NBP2-14786-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0182

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ATAD2 Antibodies »

Product Summary for ATAD2 Antibody (CL0182)

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TAYAIIKEELDEDFEQLCEEIQESRKKRGCSSSKYAPSYYHVMPKQNSTLVGDKRSDPEQNEKLKTPSTPVACSTPAQLKRKIRKKSNWYLGTIKKRRKISQAKDDSQNAIDHK

Epitope

VGDKRSDPEQ

Reactivity Notes

Expected species cross reactivity based on sequence identity: Mouse( 82%), Rat (82%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ATAD2 Antibody (CL0182)

Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786]

Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786]

Immunohistochemistry-Paraffin: ATAD2 Antibody (CL0182) [NBP2-14786] - Staining in human testis and skeletal muscle tissues. Corresponding ATAD2 RNA-seq data are presented for the same tissues.
Western Blot: ATAD2 Antibody (CL0182) [NBP2-14786]

Western Blot: ATAD2 Antibody (CL0182) [NBP2-14786]

Western Blot: ATAD2 Antibody (CL0182) [NBP2-14786] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATAD2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: ATAD2 Antibody (CL0182) [NBP2-14786]

Immunocytochemistry/ Immunofluorescence: ATAD2 Antibody (CL0182) [NBP2-14786]

Immunocytochemistry/Immunofluorescence: ATAD2 Antibody (CL0182) [NBP2-14786] - Staining of MCF7 cells using the Anti-ATAD2 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
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Applications for ATAD2 Antibody (CL0182)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Simple Western

1:20

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATAD2

ATAD2 is a large family of ATPases has been described, whose key feature is that they share a conserved region of about 220 amino acids that contains an ATP-binding site. The proteins that belong to this family either contain one or two AAA (ATPases Associated with diverse cellular Activities) domains. AAA family proteins often perform chaperone-like functions that assist in the assembly, operation, or disassembly of protein complexes. The protein encoded by this gene contains two AAA domains, as well as a bromodomain. [provided by RefSeq]

Long Name

ATPase family, AAA domain containing 2

Alternate Names

ANCCA, CT137, PRO2000

Gene Symbol

ATAD2

Additional ATAD2 Products

Product Documents for ATAD2 Antibody (CL0182)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ATAD2 Antibody (CL0182)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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