ATAD2 Antibody (JE34-52)

Western Blot: ATAD2 Antibody (JE34-52) [NBP3-32052]

Western Blot: ATAD2 Antibody (JE34-52) [NBP3-32052] - Western blot analysis of ATAD2 on different lysates with Rabbit anti-ATAD2 antibody (NBP3-32052) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: MCF7 cell lysate
Lane 3: T-47D cell lysate
Lane 4: HT-29 cell lysate
Lane 5: Saos- 2 cell lysate
Lane 6: HepG2 cell lysate
Lane 7: Jurkat cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 158 kDa
Observed band size: 180 kDa

Exposure time: 4 minutes 41 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32052) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: ATAD2 Antibody (JE34-52) [NBP3-32052]

Immunohistochemistry: ATAD2 Antibody (JE34-52) [NBP3-32052] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-ATAD2 antibody (NBP3-32052) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32052) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.