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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 103C411.2

Format

Azide Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was generated by immunizing mice with a synthetic peptide containing phosphorylated threonine at position 71 of human ATF-2.

Modification

p Thr71

Specificity

This monoclonal antibody is specific for phospho (Thr71) ATF2. It cannot be used to test for non-phosphorylated ATF2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ATF2 [p Thr71] Antibody (103C411.2) - Azide Free

Western Blot: ATF2 [p Thr71] Antibody (103C411.2) - Azide Free [NBP2-33236] - Detection of ATF2 phosphorylation in HeLa cell lysate with phospho ATF2 antibody. Lane A. Untreated cell lysate; Lane B. HeLa cells treated with TNF for 1 hr. Image from the standard format of this antibody.

Applications for ATF2 [p Thr71] Antibody (103C411.2) - Azide Free

Application
Recommended Usage

Flow Cytometry

1ul/1 million cells

Western Blot

2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS - BSA free

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATF2

ATF-2 (activating transcription factor-2, CRE-BP1) is a transcription factor that binds the cAMP response element (CRE) and AP-1 response element and is abundantly expressed in the brain. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2 (2). SAPK and p38 MAPK can activate, in response to cellular stress, ATF-2 by phosphorylating this protein at Thr69 and Thr71. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4).

Long Name

Activating Transcription Factor 2

Alternate Names

CRE-BP1, CREB2, HB16, mXBP, TREB7

Gene Symbol

ATF2

Additional ATF2 Products

Product Documents for ATF2 [p Thr71] Antibody (103C411.2) - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ATF2 [p Thr71] Antibody (103C411.2) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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