ATF2 [p Thr71] Antibody (4F2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26431

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26431-50ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4F2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ATF2 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human ATF2 [p Thr71] [UniProt P15336]

Modification

p Thr 71

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATF2 [p Thr71] Antibody (4F2)

ATF2 [p Thr71] Antibody (4F2)

Western Blot: ATF2 [p Thr71] Antibody (4F2) [NBP3-26431] -

Western Blot: ATF2 [p Thr71] Antibody (4F2) [NBP3-26431] - Positive Western Blot detected in 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate (treated with Calyculin A or EGF).
All lanes: ATF2 [p Thr71] Antibody at 1.015ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 70 KDa
Observed band size: 70 KDa
ATF2 [p Thr71] Antibody (4F2)

Immunocytochemistry/Immunofluorescence: ATF2 [p Thr71] Antibody (4F2) [NBP3-26431] -

Immunocytochemistry/Immunofluorescence: ATF2 [p Thr71] Antibody (4F2) [NBP3-26431] - Staining of A549 cells(treated with 100mM EGF for 20min) with ATF2 [p Thr71] Antibody (4F2) at 1:63, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for ATF2 [p Thr71] Antibody (4F2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: ATF2

ATF-2 (activating transcription factor-2, CRE-BP1) is a transcription factor that binds the cAMP response element (CRE) and AP-1 response element and is abundantly expressed in the brain. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2 (2). SAPK and p38 MAPK can activate, in response to cellular stress, ATF-2 by phosphorylating this protein at Thr69 and Thr71. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4).

Long Name

Activating Transcription Factor 2

Alternate Names

CRE-BP1, CREB2, HB16, mXBP, TREB7

Gene Symbol

ATF2

Additional ATF2 Products

Product Documents for ATF2 [p Thr71] Antibody (4F2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ATF2 [p Thr71] Antibody (4F2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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