ATF2 [p Thr71] Antibody (SR2159)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21538

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21538-100ul
NBP3-21538-50ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2159

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ATF2 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human phospho-ATF2 [Thr71] (Uniprot #: P15336)

Modification

p Thr 71

Specificity

Detects endogenous levels of phospho-ATF2 [Thr71]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATF2 [p Thr71] Antibody (SR2159)

Western Blot: ATF2 [p Thr 71] Antibody (SR2159) [NBP3-21538] -

Western Blot: ATF2 [p Thr 71] Antibody (SR2159) [NBP3-21538] - Western blot analysis using NBP3-21538 in (1) HeLa cell lysate; (2) HeLa cell lysate treated with Anisomycin.

Applications for ATF2 [p Thr71] Antibody (SR2159)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunoprecipitation

1:30

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATF2

ATF-2 (activating transcription factor-2, CRE-BP1) is a transcription factor that binds the cAMP response element (CRE) and AP-1 response element and is abundantly expressed in the brain. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2 (2). SAPK and p38 MAPK can activate, in response to cellular stress, ATF-2 by phosphorylating this protein at Thr69 and Thr71. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4).

Long Name

Activating Transcription Factor 2

Alternate Names

CRE-BP1, CREB2, HB16, mXBP, TREB7

Gene Symbol

ATF2

Additional ATF2 Products

Product Documents for ATF2 [p Thr71] Antibody (SR2159)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATF2 [p Thr71] Antibody (SR2159)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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