ATF2 [p Thr69] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-81798

Novus Biologicals, part of Bio-Techne
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NB100-81798
NB100-81798-0.025ml
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ATF2 Antibodies »

Product Specifications

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human ATF2 around the phosphorylation site of threonine 69 or 51 (D-Q-TP-P-T).

Modification

p Thr69

Specificity

Detects endogenous levels of ATF2 only when phosphorylated at threonine 69 or 51.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATF2 [p Thr69] Antibody

Western Blot: ATF2 [p Thr69] Antibody [NB100-81798]

Western Blot: ATF2 [p Thr69] Antibody [NB100-81798]

Western Blot: ATF2 [p Thr69] Antibody [NB100-81798] - Analysis of extracts from 3T3 cells untreated or treated with Anisomycin using ATF2 (Phospho-Thr69 or 51) antibody

Immunocytochemistry/Immunofluorescence: ATF2 [p Thr69] Antibody [NB100-81798] -

Immunocytochemistry/Immunofluorescence: ATF2 [p Thr69] Antibody [NB100-81798] - Immunofluorescence staining of methanol-fixed Hela cells using NB100-81798

Immunohistochemistry-Paraffin: ATF2 [p Thr69] Antibody [NB100-81798] -

Immunohistochemistry-Paraffin: ATF2 [p Thr69] Antibody [NB100-81798] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NB100-81798 (left) or the same antibody preincubated with blocking peptide (right).
View 1 more images

Applications for ATF2 [p Thr69] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:200

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50 - 1:100

Western Blot

1:500

Reviewed Applications

Read 1 review rated 4 using NB100-81798 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATF2

ATF2 (Activating Transcription Factor 2, CREBP, HB16, CREB2, TREB7) is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.

Long Name

Activating Transcription Factor 2

Alternate Names

CRE-BP1, CREB2, HB16, mXBP, TREB7

Entrez Gene IDs

1386 (Human); 11909 (Mouse); 81647 (Rat)

Gene Symbol

ATF2

UniProt

P15336

Additional ATF2 Products

Product Documents for ATF2 [p Thr69] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ATF2 [p Thr69] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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