ATF3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92402

Novus Biologicals, part of Bio-Techne
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NBP2-92402-0.02ml
NBP2-92402-0.1ml
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ATF3 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-181 of human ATF3 (NP_001665.1). MMLQHPGQVSASEVSASAIVPCLSPPGSLVFEDFANLTPFVKEELRFAIQNKHLCHRMSSALESVTVSDRPLGVSITKAEVAPEEDERKKRRRERNKIAAAKCRNKKKEKTECLQKESEKLESVNAELKAQIEELKNEKQHLIYMLNLHRPTCIVRAQNGRTPEDERNLFIQQIKEGTLQS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATF3 Antibody - BSA Free

ATF3 Antibody - BSA Free

Western Blot: ATF3 Antibody - BSA Free [NBP2-92402] -

Western Blot: ATF3 Antibody - BSA Free [NBP2-92402] - Western blot analysis of various lysates using ATF3 Rabbit pAb at 1:1000 dilution incubated overnight at 4C.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit
Exposure time: 120 s.
ATF3 Antibody - BSA Free

Western Blot: ATF3 Antibody - BSA Free [NBP2-92402] -

Western Blot: ATF3 Antibody - BSA Free [NBP2-92402] - Western blot analysis of lysates from RAW 264.7 cells using ATF3 Rabbit pAb at 1:1000 dilution incubated overnight at 4C. Raw264.7 cells were treated by LPS (1 ug/ml) at 37C for 8 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 30 ug per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit
Exposure time: 180 s.

Applications for ATF3 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATF3

Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes.

Alternate Names

activating transcription factor 3cAMP-dependent transcription factor ATF-3, ATF3deltaZip2, ATF3deltaZip2c, ATF3deltaZip3, cyclic AMP-dependent transcription factor ATF-3

Gene Symbol

ATF3

Additional ATF3 Products

Product Documents for ATF3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ATF3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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