ATF4 Antibody (SD20-92)

Flow Cytometry: ATF4 Antibody (SD20-92) [NBP2-67766]

Flow Cytometry: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of Hela cells with ATF4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766]

Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human liver carcinoma tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766]

Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human prostate carcinoma tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766]

Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human skin tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766]

Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766]

Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human stomach carcinoma tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766]

Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human colon carcinoma tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766]

Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human thyroid tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766]

Immunohistochemistry-Paraffin: ATF4 Antibody (SD20-92) [NBP2-67766] - Analysis of paraffin-embedded human small intestine tissue using anti-ATF4 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.