ATF5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51908

Novus Biologicals, part of Bio-Techne
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NBP1-51908
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all ATF5 Antibodies »

Product Specifications

Immunogen

Peptide with sequence EVYKARSQRTRSC corresponding to C-Terminus according to NP_036200.2.

Epitope

EVYKARSQRTRSC

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ATF5 Antibody

Western Blot: ATF5 Antibody [NBP1-51908]

Western Blot: ATF5 Antibody [NBP1-51908]

Western Blot: ATF5 Antibody [NBP1-51908] - Staining of Mouse (A) and Rat (B) Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: ATF5 Antibody [NBP1-51908]

Western Blot: ATF5 Antibody [NBP1-51908]

Western Blot: ATF5 Antibody [NBP1-51908] - Analysis of ATF5 antibody in human heart lysate (RIPA buffer, 35 ug total loaded per lane) using NBP1-51908 at 1.5 ug/ml. Primary incubated for 1 hour. ATF5 antibody detected by western blot using chemiluminescence.

Applications for ATF5 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 30 kDa band observed in human heart lysates (calculated MW of 30.7 kDa band according to NP_036200.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATF5

Transcriptional activator which binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters and blocks the differentiation of neuroprogenitor cells into neurons. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4

Alternate Names

activating transcription factor 5FLJ34666, ATFXHMFN0395, cAMP-dependent transcription factor ATF-5, cyclic AMP-dependent transcription factor ATF-5, Transcription factor ATFx

Entrez Gene IDs

22809 (Human); 107503 (Mouse); 282840 (Rat)

Gene Symbol

ATF5

UniProt

NP_036200.2 (Human)

Additional ATF5 Products

Product Documents for ATF5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATF5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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