Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Orangutan (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ATF7 Antibodies »

Product Summary for ATF7 Antibody

Immunogen

maps to a region between residue 444 and 494 of human activating transcription factor 7 using the numbering given in entry CAA40483.1

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATF7 Antibody

Immunoprecipitation: ATF7 Antibody [NBP1-30071]

Immunoprecipitation: ATF7 Antibody [NBP1-30071]

Immunoprecipitation: ATF7 Antibody [NBP1-30071] - Whole cell lysate from HeLa cells. ATF7 was also immunoprecipitated by rabbit anti-ATF7 antibody. For blotting immunoprecipitated ATF7, the ReliaBLOT(R) Reagents and Procedures were used.

Applications for ATF7 Antibody

Application
Recommended Usage

Immunoprecipitation

10-15 ug/mg lysate

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ATF7

Eukaryotic gene transcription is regulated by sequence-specific transcription factors, which bind modular cis acting promoter and enhancer elements. The cAMP response element (CRE) consists of the palindromic octanucleotide TGACGTCA. There are several CRE binding proteins within the ATF/CREB family, including CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. A novel basic leucine zipper (bZIP) protein, designated ATF-7, is closely related to members of the ATF/CREB family of bZIP proteins, with highest homology to ATF-4. ATF-7 physically interacts with the PRL-1 protein-tyrosine phosphatase (PTPase), which is a predominately nuclear, farnesylated PTPase. ATF-7 homodimers bind specifically to CRE elements. ATF-7 is expressed in a number of different tissues and is expressed in association with differentiation. ATF-7 and PRL-1 interact with each other through the bZIP region of ATF-7 and the phosphatase domain of PRL-1. In addition, PRL-1 is able to dephosphorylate ATF-7 in vitro.

Alternate Names

activating transcription factor 7ATFATranscription factor ATF-A, cAMP-dependent transcription factor ATF-7, cyclic AMP-dependent transcription factor ATF-7, MGC57182

Gene Symbol

ATF7

Additional ATF7 Products

Product Documents for ATF7 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATF7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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