ATG10 Antibody (2Q3Q3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16649

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2Q3Q3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ATG10 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 121-220 of human ATG10 (Q9H0Y0). RPLTLKDIWEGVHECYKMRLLQGPWDTITQQEHPILGQPFFVLHPCKTNEFMTPVLKNSQKINKNVNYITSWLSIVGPVVGLNLPLSYAKATSQDERNVP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATG10 Antibody (2Q3Q3)

Western Blot: ATG10 Antibody (2Q3Q3) [NBP3-16649]

Western Blot: ATG10 Antibody (2Q3Q3) [NBP3-16649]

Western Blot: ATG10 Antibody (2Q3Q3) [NBP3-16649] - Western blot analysis of extracts of various cell lines, using ATG10 Rabbit mAb (NBP3-16649) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: ATG10 Antibody (2Q3Q3) [NBP3-16649]

Immunocytochemistry/ Immunofluorescence: ATG10 Antibody (2Q3Q3) [NBP3-16649]

Immunocytochemistry/Immunofluorescence: ATG10 Antibody (2Q3Q3) [NBP3-16649] - Immunofluorescence analysis of NIH-3T3 cells using ATG10 Rabbit mAb (NBP3-16649) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for ATG10 Antibody (2Q3Q3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATG10

Atg10 is a major contributor to cellular homeostasis is the ability of the cell to strike a balance between the formation and degradation/removal of its cellular components. This process of internal cellular turn-over is called autophagy (self-eating), and is facilitated by a pathway of around 16 interacting proteins in the human. APG10 interacts with LC3A to facilitate the conjugation of ATG12 to ATG5. APG10 is also able to directly interact either with ATG5 or ATG7A. A disruption to the autophagic process is now associated with the progression of several cancers, neurodegenerative disorders and cardiac pathologies.

Long Name

Autophagy Related 10 Homolog

Alternate Names

APG10, APG10L, PP12616

Gene Symbol

ATG10

Additional ATG10 Products

Product Documents for ATG10 Antibody (2Q3Q3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATG10 Antibody (2Q3Q3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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