ATG10 Antibody (JE59-86)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32055
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE59-86
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide corresponding to Human ATG10 aa 180-220 (C terminal) (Uniprot: Q9H0Y0)
Localization
Cytoplasm.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ATG10 Antibody (JE59-86)
Western Blot: ATG10 Antibody (JE59-86) [NBP3-32055]
Western Blot: ATG10 Antibody (JE59-86) [NBP3-32055] - Western blot analysis of ATG10 on different lysates with Rabbit anti-ATG10 antibody (NBP3-32055) at 1/1,000 dilution.Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: K-562 cell lysate
Lysates/proteins at 10 ug/Lane.
Predicted band size: 25 kDa
Observed band size: 30 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32055) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Applications for ATG10 Antibody (JE59-86)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ATG10
Long Name
Autophagy Related 10 Homolog
Alternate Names
APG10, APG10L, PP12616
Gene Symbol
ATG10
Additional ATG10 Products
Product Documents for ATG10 Antibody (JE59-86)
Product Specific Notices for ATG10 Antibody (JE59-86)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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