ATG12 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB600-603
Key Product Details
Species Reactivity
Validated:
Yeast
Cited:
Bacteria
Applications
Validated:
ELISA, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This purified ATG12 Antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast ATG12 protein. (Uniprot: P38316)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for ATG12 Antibody
![Western Blot: ATG12 Antibody [NB600-603]](https://resources.bio-techne.com/images/products/ATG12-Antibody-Western-Blot-NB600-603-img0002.jpg "Western Blot: ATG12 Antibody [NB600-603]")
Western Blot: ATG12 Antibody [NB600-603]
Western Blot: ATG12 Antibody [NB600-603] - Immunoblot of APG12 fusion protein. Anti-APG12 antibody generated by immunization with recombinant yeast APG12 was tested by immunoblot against yeast lysates expressing the APG12-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL.
N/A: ATG12 Antibody [NB600-603]
Applications for ATG12 Antibody
Application
Recommended Usage
ELISA
1:1000-1:5000
Western Blot
1:500-1:2000
Application Notes
This purified polyclonal antibody reacts yeast APG12 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates). Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 1 using NB600-603 in the following applications:
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ATG12
Long Name
ATG12 Autophagy Related 12 Homolog
Alternate Names
APG12, FBR93, HAPG12
Gene Symbol
ATG12
UniProt
Additional ATG12 Products
Product Documents for ATG12 Antibody
Product Specific Notices for ATG12 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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