Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all ATG12 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 15 amino acid synthetic peptide from near the center of human ATG12. The immunogen is located within the first 50 amino acids of ATG12. Amino Acid Squence: LTDVSPETTTPEPPS

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATG12 Antibody - BSA Free

Western Blot: ATG12 AntibodyBSA Free [NBP1-76861]

Western Blot: ATG12 AntibodyBSA Free [NBP1-76861]

Western Blot: ATG12 Antibody [NBP1-76861] - Mouse heart tissue lysate with ATG12 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
ATG12 Antibody - BSA Free

Immunohistochemistry: ATG12 Antibody - BSA Free [NBP1-76861] -

Immunohistochemistry: ATG12 Antibody - BSA Free [NBP1-76861] - Immunohistochemistry of ATG12 in human brain tissue with ATG12 antibody at 2.5 ug/mL.
ATG12 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ATG12 Antibody - BSA Free [NBP1-76861] -

Immunocytochemistry/ Immunofluorescence: ATG12 Antibody - BSA Free [NBP1-76861] - Immunofluorescence of ATG12 in Human Brain cells with ATG12 antibody at 20 ug/mL.

Applications for ATG12 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

0.5-1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATG12

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG12, another member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7. At least two isoforms of ATG12 are known to exist.

Long Name

ATG12 Autophagy Related 12 Homolog

Alternate Names

APG12, FBR93, HAPG12

Gene Symbol

ATG12

UniProt

EAW48955 (Human)

Additional ATG12 Products

Product Documents for ATG12 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATG12 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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