ATG14 Antibody (1F7C10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27119
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1F7C10
Concentration
1 mg/ml
Product Summary for ATG14 Antibody (1F7C10)
Immunogen
Purified recombinant fragment of human ATG14 (AA: 43-303) expressed in E. Coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
55.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ATG14 Antibody (1F7C10)
Western Blot: ATG14 Antibody (1F7C10) [NBP3-27119] -
Western Blot: ATG14 Antibody (1F7C10) [NBP3-27119] - Analysis using ATG14 mAb against HEK293 (1) and ATG14 (AA: 43-303)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: ATG14 Antibody (1F7C10) [NBP3-27119] -
ELISA: ATG14 Antibody (1F7C10) [NBP3-27119] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
Immunohistochemistry: ATG14 Antibody (1F7C10) [NBP3-27119] -
Immunohistochemistry: ATG14 Antibody (1F7C10) [NBP3-27119] - Analysis of paraffin-embedded testis tissues using ATG14 mouse mAb with DAB staining.Applications for ATG14 Antibody (1F7C10)
Application
Recommended Usage
ELISA
1:10000
Immunohistochemistry
1:200 - 1:1000
Immunohistochemistry-Paraffin
1:200 - 1:1000
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ATG14
Alternate Names
ATG14 Autophagy Related 14 Homolog, ATG14 Autophagy Related 14 Homolog (S. Cerevisiae), ATG14L, Autophagy Related 14, Autophagy-Related Protein 14-Like Protein, Barkor, Beclin 1-Associated Autophagy-Related Key Regulator, Beclin 1-interacting protein, KIAA0831
Gene Symbol
ATG14
Additional ATG14 Products
Product Documents for ATG14 Antibody (1F7C10)
Product Specific Notices for ATG14 Antibody (1F7C10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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