ATG16L1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-60928

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Alligator, Bovine, Canine, Primate

Cited:

Human, Mouse, Rat, Alligator

Applications

Validated:

Electron Microscopy, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all ATG16L1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide within residues 1-100 of human ATG16L1 protein. [Swiss-Prot# Q676U5]

Reactivity Notes

Use in Alligator reported in scientific literature (PMID:32061056).

Localization

Cytoplasm. Preautophagosomal structure, preautophagosomal structure membrane, peripheral membrane protein. Note: Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATG16L1 Antibody - BSA Free

Western Blot: ATG16L1 AntibodyBSA Free [NB110-60928]

Western Blot: ATG16L1 AntibodyBSA Free [NB110-60928]

Western Blot: ATG16L1 Antibody [NB110-60928] - Detection of ATG16L1 using NB110-60928 in HCT116 whole cell extracts. Image from verified customer review.
Immunohistochemistry-Paraffin: ATG16L1 Antibody - BSA Free [NB110-60928]

Immunohistochemistry-Paraffin: ATG16L1 Antibody - BSA Free [NB110-60928]

Immunohistochemistry-Paraffin: ATG16L1 Antibody [NB110-60928] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human colon cancer using ATG16L1 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of ATG16L1 was observed. Staining was performed by Histowiz.
Western Blot: ATG16L1 AntibodyBSA Free [NB110-60928]

Western Blot: ATG16L1 AntibodyBSA Free [NB110-60928]

Western Blot: ATG16L1 Antibody [NB110-60928] - Detection of ATG16L1 using NB110-60928 in HeLa whole cell extracts.

Applications for ATG16L1 Antibody - BSA Free

Application
Recommended Usage

Electron Microscopy

1:10-1:500. Use reported in scientific literature (PMID 22531915)

Immunohistochemistry

1:250 - 1:500

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 25060858)

Western Blot

0.5-2 ug/ml
Application Notes
In Western blot analysis, a specific band is seen at ~68kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 4 using NB110-60928 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATG16L1

Atg16L1 (autophagy-related protein 16-1) forms a component of a protein complex that directs LC3 to autophagosomes en route to their fusion with lysosomes, thereby playing a key role in the process of autophagosome maturation. Atg16L1 belongs to WD repeat ATG16 family, exist as homooligomer and interacts with ATG5 as well as RAB33B, and forms a part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kD) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kD). It is found in cytoplasm and gets localized to preautophagosomal structure where it is implicated in the membrane targeting of ATG5. Mice deficient in Atg16L1 (Atg16L1HM) display reduced autophagy and develop, on viral infection, intestinal abnormalities similar to pathologies found in Crohn's disease patients, including abnormalities in the integrity, architecture, and function of Paneth cells, which are specialized secretory epithelial cells of the small intestine. Atg16L1 has also been shown to play a role in modulating proinflammatory responses and population genetic studies have positively linked a common polymorphism in ATG16L1 gene with inflammatory bowel disease type 10 (IBD10).

Alternate Names

APG16 autophagy 16-like (S. cerevisiae), APG16L beta, APG16LFLJ10828, APG16-like 1, ATG16 autophagy related 16-like (S. cerevisiae), ATG16 autophagy related 16-like 1 (S. cerevisiae), ATG16A, ATG16L, autophagy-related protein 16-1, FLJ00045, FLJ10035, FLJ22677, IBD10, WD repeat domain 30, WDR30

Entrez Gene IDs

55054 (Human); 77040 (Mouse); 363278 (Rat)

Gene Symbol

ATG16L1

UniProt

Q676U5 (Human)

Additional ATG16L1 Products

Product Documents for ATG16L1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ATG16L1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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