ATG4B Antibody (PSH02-53)

Western Blot: ATG4B Antibody (PSH02-53) [NBP3-32058]

Western Blot: ATG4B Antibody (PSH02-53) [NBP3-32058] - Western blot analysis of ATG4B on different lysates with Rabbit anti-ATG4B antibody (NBP3-32058) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: 293T cell lysate
Lane 4: MCF7 cell lysate
Lane 5: K-562 cell lysate
Lane 6: COS-1 cell lysate
Lane 7: NIH/3T3 cell lysate
Lane 8: C2C12 cell lysate
Lane 9: PC-12 cell lysate
Lane 10: Mouse brain tissue lysate
Lane 11: Rat brain tissue lysate
Lane 12: Mouse kidney tissue lysate
Lane 13: Mouse colon tissue lysate

Lysates/proteins at 30 µg/Lane.

Predicted band size: 44 kDa
Observed band size: 47 kDa

Exposure time: 42 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.