ATG4D Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-36939
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence CSQLAQPTLRLPRT corresponding to internal region (near C-Terminus) according to NP_116274.3.
Epitope
internal region (near C terminus)
Reactivity Notes
Expected from sequence similarity: Mouse, Rat, Dog, Pig
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for ATG4D Antibody
Immunocytochemistry/Immunofluorescence: Goat Polyclonal ATG4D Antibody [NBP1-36939] -
Immunocytochemistry/Immunofluorescence: Goat Polyclonal ATG4D Antibody [NBP1-36939] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).![Immunohistochemistry-Paraffin: ATG4D Antibody [NBP1-36939]](https://resources.bio-techne.com/images/products/ATG4D-Antibody-Immunohistochemistry-Paraffin-NBP1-36939-img0001.jpg "Immunohistochemistry-Paraffin: ATG4D Antibody [NBP1-36939]")
Immunohistochemistry-Paraffin: ATG4D Antibody [NBP1-36939]
Immunohistochemistry-Paraffin: ATG4D Antibody [NBP1-36939] - Staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: Goat Polyclonal ATG4D Antibody [NBP1-36939] -
Flow Cytometry: Goat Polyclonal ATG4D Antibody [NBP1-36939] - Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Applications for ATG4D Antibody
Application
Recommended Usage
ELISA
Detection limit 1:32000
Flow Cytometry
10 ug/ml
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Immunohistochemistry
2.5 ug/ml
Immunohistochemistry-Paraffin
2.5 ug/ml
Application Notes
Western blot: Preliminary experiments gave an approx. 26kDa band in Human Skeletal Muscle lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 52.9kDa according to NP_116274.3. The 26kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Pancreas
IHC: Paraffin embedded Human Pancreas
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ATG4D
Alternate Names
APG4 autophagy 4 homolog D, APG4 autophagy 4 homolog D (S. cerevisiae), APG4D, APG4-D, ATG4 autophagy related 4 homolog D (S. cerevisiae), AUTL4, AUT-like 4 cysteine endopeptidase, AUT-like 4, cysteine endopeptidase, AUT-like 4, cysteine endopeptidase (S. cerevisiae), Autophagin-4, Autophagy-related cysteine endopeptidase 4, Autophagy-related protein 4 homolog D, cysteine protease ATG4D, cysteine protease involved in autophagy, EC 3.4.22, EC 3.4.22.-
Gene Symbol
ATG4D
UniProt
Additional ATG4D Products
Product Documents for ATG4D Antibody
Product Specific Notices for ATG4D Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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