ATG4D Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-41308

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all ATG4D Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 17 amino acid peptide near the amino terminus of human ATG4D. The immunogen is located within the first 50 amino acids of ATG4D. Amino Acid Squence: SPEDARRRPEARRPRG

Specificity

At least two isoforms of ATG4D are known to exist; this antibody will detect only the larger isoform. ATG4D is predicted to not cross-react with other ATG4 proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATG4D Antibody - BSA Free

Western Blot: ATG4D AntibodyBSA Free [NBP2-41308]

Western Blot: ATG4D AntibodyBSA Free [NBP2-41308]

Western Blot: ATG4D Antibody [NBP2-41308] - Analysis of ATG4D in human testis tissue lysate with ATG4D antibody at (A) 1 and (B) 2 ug/ml.
Immunohistochemistry: ATG4D Antibody - BSA Free [NBP2-41308]

Immunohistochemistry: ATG4D Antibody - BSA Free [NBP2-41308]

Immunohistochemistry: ATG4D Antibody [NBP2-41308] - Human testis tissue with ATG4D antibody at 20 ug/ml.
Immunohistochemistry: ATG4D Antibody - BSA Free [NBP2-41308]

Immunohistochemistry: ATG4D Antibody - BSA Free [NBP2-41308]

Immunohistochemistry: ATG4D Antibody [NBP2-41308] - Immunohistochemistry of ATG4D in human testis tissue with ATG4D antibody at 5 ug/ml.

Applications for ATG4D Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATG4D

ATG4D is a gene that codes for a protein expressed in the skeletal muscle and testis and is 474 amino acids long with a weight of approximately 53 kDa. ATG4D is a cysteine protease required for autophagy. ATG4D has been shown to have interactions with TAF12, CASP3, CASP8, GABARAP, and UBC in pathways such as the regulation of autophagy pathway.

Alternate Names

APG4 autophagy 4 homolog D, APG4 autophagy 4 homolog D (S. cerevisiae), APG4D, APG4-D, ATG4 autophagy related 4 homolog D (S. cerevisiae), AUTL4, AUT-like 4 cysteine endopeptidase, AUT-like 4, cysteine endopeptidase, AUT-like 4, cysteine endopeptidase (S. cerevisiae), Autophagin-4, Autophagy-related cysteine endopeptidase 4, Autophagy-related protein 4 homolog D, cysteine protease ATG4D, cysteine protease involved in autophagy, EC 3.4.22, EC 3.4.22.-

Gene Symbol

ATG4D

Additional ATG4D Products

Product Documents for ATG4D Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATG4D Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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