ATG5 Antibody (ATG5/2101) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08504
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry-Paraffin, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # ATG5/2101
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant fragment of human ATG5 protein (around aa 1-119) (exact sequence is proprietary) (Uniprot: Q9H1Y0)
Localization
Cytoplasm
Marker
Autophagy Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07889).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for ATG5 Antibody (ATG5/2101) - Azide and BSA Free
Western Blot: ATG5 Antibody (ATG5/2101)Azide and BSA Free [NBP3-08504]
Western Blot: ATG5 Antibody (ATG5/2101) [NBP3-08504] - Western Blot Analysis of THP-1 cell and human Brain tissue lysate using ATG5 Mouse Monoclonal Antibody (ATG5/2101).Immunohistochemistry-Paraffin: ATG5 Antibody (ATG5/2101) - Azide and BSA Free [NBP3-08504]
Immunohistochemistry-Paraffin: ATG5 Antibody (ATG5/2101) [NBP3-08504] - Formalin-fixed, paraffin-embedded human Endometrium stained with ATG5 Mouse Monoclonal Antibody (ATG5/2101).Immunohistochemistry-Paraffin: ATG5 Antibody (ATG5/2101) - Azide and BSA Free [NBP3-08504]
Immunohistochemistry-Paraffin: ATG5 Antibody (ATG5/2101) [NBP3-08504] - Formalin-fixed, paraffin-embedded human Brain stained with ATG5 Mouse Monoclonal Antibody (ATG5/2101).Applications for ATG5 Antibody (ATG5/2101) - Azide and BSA Free
Application
Recommended Usage
ELISA
1-5 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
ELISA: 1-5ug/ml. Order antibody without BSA
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: ATG5
In the context of its role in autophagy, Atg5 plays diverse physiologically relevant roles. For example, Atg5 together with Atg7 are required for adipogenesis (3). Recently, Atg5 has been implicated in the process of B-cell receptor polarization and antigen presentation (4). In addition to its role in autophagy, Atg5 is implicated in apoptotic cell death. Interaction of Atg5 with FADD (Fas-associated protein with death domain) is involved in cell death induced by IFN-gamma. A truncated form of Atg5, a 24kDa fragment, leads to cell death by interacting with Bcl-xl and inhibiting its anti-apoptotic activity (5). Other Atg5 interacting partners include interleukin-beta (IL-beta) converting enzyme and nucleotide binding oligomerization domain protein 1, which suggest that Atg5 may play other biologically relevant roles (3).
References
1. Yang, Z., & Klionsky, D. J. (2010). Mammalian autophagy: Core molecular machinery and signaling regulation. Current Opinion in Cell Biology. https://doi.org/10.1016/j.ceb.2009.11.014
2. Rubinsztein, D. C., Shpilka, T., & Elazar, Z. (2012). Mechanisms of autophagosome biogenesis. Current Biology. https://doi.org/10.1016/j.cub.2011.11.034
3. Subramani, S., & Malhotra, V. (2013). Non-autophagic roles of autophagy-related proteins. EMBO Reports. https://doi.org/10.1038/embor.2012.220
4. Arbogast, F., Arnold, J., Hammann, P., Kuhn, L., Chicher, J., Murera, D., Gros, F. (2019). ATG5 is required for B cell polarization and presentation of particulate antigens. Autophagy. https://doi.org/10.1080/15548627.2018.1516327
5. Luo, S., & Rubinsztein, D. C. (2007). Atg5 and Bcl-2 provide novel insights into the interplay between apoptosis and autophagy. Cell Death and Differentiation. https://doi.org/10.1038/sj.cdd.4402149
Long Name
ATG5 Autophagy Related 5 Homolog
Alternate Names
APG5, ASP
Gene Symbol
ATG5
Additional ATG5 Products
Product Documents for ATG5 Antibody (ATG5/2101) - Azide and BSA Free
Product Specific Notices for ATG5 Antibody (ATG5/2101) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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