ATG7 Antibody (3X1W4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15808

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15808-20ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3X1W4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ATG7 Antibodies »

Product Summary for ATG7 Antibody (3X1W4)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Atg7(Apg7) (O95352). MAAATGDPGLSKLQFAPFSSALDVGFWHELTQKKLNEYRLDEAPKDIKGYYYNGDSAGLPARLTLEFSAFDMSAPTPARCCPAIGTLYNTNTLESFKTAD

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATG7 Antibody (3X1W4)

Western Blot: ATG7 Antibody (3X1W4) [NBP3-15808]

Western Blot: ATG7 Antibody (3X1W4) [NBP3-15808]

Western Blot: ATG7 Antibody (3X1W4) [NBP3-15808] - Analysis of extracts of various cell lines, using ATG7 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: ATG7 Antibody (3X1W4) [NBP3-15808]

Immunohistochemistry-Paraffin: ATG7 Antibody (3X1W4) [NBP3-15808]

Immunohistochemistry-Paraffin: ATG7 Antibody (3X1W4) [NBP3-15808] - Rat spleen using ATG7 antibody (NBP3-15808) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: ATG7 Antibody (3X1W4) [NBP3-15808]

Immunohistochemistry-Paraffin: ATG7 Antibody (3X1W4) [NBP3-15808]

Immunohistochemistry-Paraffin: ATG7 Antibody (3X1W4) [NBP3-15808] - Human esophageal cancer using ATG7 antibody (NBP3-15808) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for ATG7 Antibody (3X1W4)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:50 -1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATG7

FUNCTION: Functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1 (GABARAPL2, GABARAP or MAP1ALC3). Formation of the final GABARAPL1-PE conjugate is essential for autophagy. SUBUNIT: Homodimer (By similarity). Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. SUBCELLULAR LOCATION: Cytoplasm (Probable). ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. DOMAIN: The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12. SIMILARITY: Belongs to the ATG7 family.

Long Name

ATG7 Autophagy Related 7 Homolog

Alternate Names

APG7, APG7L, GSA7

Gene Symbol

ATG7

Additional ATG7 Products

Product Documents for ATG7 Antibody (3X1W4)

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Product Specific Notices for ATG7 Antibody (3X1W4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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