ATG7 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76649

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Chicken (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all ATG7 Antibodies »

Product Summary for ATG7 Antibody - BSA Free

Immunogen

Antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human APG7. The immunogen is located within amino acids 590 - 640 of APG7. Amino Acid Squence: SDDRMNEPPTSLGLVPH

Specificity

At least three isoforms of APG7 are known to exist; this antibody will detect all three isoforms. APG7 antibody is predicted not to cross-react with other ATG family proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATG7 Antibody - BSA Free

Western Blot: ATG7 AntibodyBSA Free [NBP1-76649]

Western Blot: ATG7 AntibodyBSA Free [NBP1-76649]

Western Blot: ATG7 Antibody [NBP1-76649] - Analysis of ATG7 in Caco-2 cell lysate with ATG7 antibody using IMG-5412 at (A) 0.5, (B) 1 and (C) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: ATG7 Antibody - BSA Free [NBP1-76649]

Immunocytochemistry/ Immunofluorescence: ATG7 Antibody - BSA Free [NBP1-76649]

Immunocytochemistry/Immunofluorescence: ATG7 Antibody [NBP1-76649] - MCF7 cells.Dilution 20 ug/mL.
Immunocytochemistry/ Immunofluorescence: ATG7 Antibody - BSA Free [NBP1-76649]

Immunocytochemistry/ Immunofluorescence: ATG7 Antibody - BSA Free [NBP1-76649]

Immunocytochemistry/Immunofluorescence: ATG7 Antibody [NBP1-76649] - MCF7 cells with APG7 antibody at 10 ug/ml.

Applications for ATG7 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10-20 ug/ml

Western Blot

0.5-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATG7

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog. In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death.

Long Name

ATG7 Autophagy Related 7 Homolog

Alternate Names

APG7, APG7L, GSA7

Gene Symbol

ATG7

UniProt

NP_006386 (Human)

Additional ATG7 Products

Product Documents for ATG7 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATG7 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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