ATIC Antibody (OTI1D2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01941
![Western Blot: ATIC Antibody (OTI1D2) [NBP2-01941]](https://resources.bio-techne.com/images/products/ATIC-Antibody-OTI1D2-Western-Blot-NBP2-01941-img0003.jpg "Western Blot: ATIC Antibody (OTI1D2) [NBP2-01941]")
![Western Blot: ATIC Antibody (OTI1D2) [NBP2-01941]](https://resources.bio-techne.com/images/products/ATIC-Antibody-OTI1D2-Western-Blot-NBP2-01941-img0004.jpg "Western Blot: ATIC Antibody (OTI1D2) [NBP2-01941]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Canine, Monkey
Applications
Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI1D2
Concentration
0.71 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human ATIC(NP_004035) produced in HEK293T cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
64.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ATIC Antibody (OTI1D2)
Western Blot: ATIC Antibody (OTI1D2) [NBP2-01941]
Western Blot: ATIC Antibody (OTI1D2) [NBP2-01941] - Blot showing the effect of hypoxia on the protein expression levels of the purine biosynthetic enzymes. HIF-1 alpha is stabilized in hypoxia as expected, and no significant increase in the purine enzymes was detected between normoxic (21% oxygen) and hypoxic (1% oxygen) growth conditions. The positions of molecular markers surrounding each band of interest are shown for each blot. ADSL (NBP2-03107), ATIC (NBP2-01941), FGAMS (NBP1-84691), GART (H00002618-M01), HIF-1a (NB100-449), PAICS (NBP2-02817), PPAT (NBP2-02056). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32439803/) licensed under a CC-BY license.![Flow Cytometry: ATIC Antibody (OTI1D2) [NBP2-01941]](https://resources.bio-techne.com/images/products/ATIC-Antibody-OTI1D2-Flow-Cytometry-NBP2-01941-img0001.jpg "Flow Cytometry: ATIC Antibody (OTI1D2) [NBP2-01941]")
Flow Cytometry: ATIC Antibody (OTI1D2) [NBP2-01941]
Flow Cytometry: ATIC Antibody (OTI1D2) [NBP2-01941] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ATIC antibody, and then analyzed by flow cytometry.![Western Blot: ATIC Antibody (OTI1D2) [NBP2-01941]](https://resources.bio-techne.com/images/products/ATIC-Antibody-OTI1D2-Western-Blot-NBP2-01941-img0002.jpg "Western Blot: ATIC Antibody (OTI1D2) [NBP2-01941]")
Western Blot: ATIC Antibody (OTI1D2) [NBP2-01941]
Western Blot: ATIC Antibody (OTI1D2) [NBP2-01941] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATIC (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATIC.Applications for ATIC Antibody (OTI1D2)
Application
Recommended Usage
Flow Cytometry
1:100
Western Blot
1:500-2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.71 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ATIC
Alternate Names
5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMPcyclohydrolase, 5-aminoimidazole-4-carboxamide-1-beta-D-ribonucleotidetransformylase/inosinicase, AICAR, AICAR formyltransferase/IMP cyclohydrolase bifunctional enzyme, AICARFT, AICARFT/IMPCHASE, bifunctional purine biosynthesis protein PURH, IMPCHASE, phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase, PURHFLJ93545
Entrez Gene IDs
471 (Human)
Gene Symbol
ATIC
Additional ATIC Products
Product Documents for ATIC Antibody (OTI1D2)
Product Specific Notices for ATIC Antibody (OTI1D2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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