Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Chicken (100%), Orangutan (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ATIC Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 50 to 100 of human 5-aminoimidazole-4-carboxamide Ribonucleotide Formyltransferase/IMP Cyclohydrolase using the numbering given in entry NP_004035.2 (GeneID 471).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATIC Antibody

Western Blot: ATIC Antibody [NBP2-32183]

Western Blot: ATIC Antibody [NBP2-32183]

Western Blot: ATIC Antibody [NBP2-32183] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-ATIC antibody NBP2-32183 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: ATIC Antibody [NBP2-32183]

Immunoprecipitation: ATIC Antibody [NBP2-32183]

Immunoprecipitation: ATIC Antibody [NBP2-32183] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-ATIC antibody NBP2-32183 used for IP at 6 ug per reaction. For blotting immunoprecipitated ATIC, NBP2-32183 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for ATIC Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ATIC

ATIC is a 5 aminoimidazole 4 carboxamide ribonucleotide formyltransferase which helps catalyze the penultimate and final steps of the de novo purine biosynthetic pathway. The human ATIC cDNA encodes a deduced 591 amino acid protein that is 81% identical to the chicken sequence.

Alternate Names

5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMPcyclohydrolase, 5-aminoimidazole-4-carboxamide-1-beta-D-ribonucleotidetransformylase/inosinicase, AICAR, AICAR formyltransferase/IMP cyclohydrolase bifunctional enzyme, AICARFT, AICARFT/IMPCHASE, bifunctional purine biosynthesis protein PURH, IMPCHASE, phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase, PURHFLJ93545

Gene Symbol

ATIC

UniProt

NP_004035.2 (Human)

Additional ATIC Products

Product Documents for ATIC Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ATIC Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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