ATP5A Antibody (5G11)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26491
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5G11
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human ATP5A [UniProt P25705]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ATP5A Antibody (5G11)
Immunohistochemistry: ATP5A Antibody (5G11) [NBP3-26491] -
Immunohistochemistry: ATP5A Antibody (5G11) [NBP3-26491] - Image of ATP5A Antibody (5G11) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: ATP5A Antibody (5G11) [NBP3-26491] -
Immunohistochemistry: ATP5A Antibody (5G11) [NBP3-26491] - Image of ATP5A Antibody (5G11) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: ATP5A Antibody (5G11) [NBP3-26491] -
Western Blot: ATP5A Antibody (5G11) [NBP3-26491] - Positive Western Blot detected in: HepG2 whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HL60 whole cell lysate, Mouse Heart tissue, Mouse Brain tissue.All lanes: ATP5A Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 60, 55, 58 kDa
Observed band size: 60 kDa
Applications for ATP5A Antibody (5G11)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: ATP5A
Long Name
ATP5A1
Alternate Names
ATP synthase alpha chain, mitochondrial, ATP synthase subunit alpha, mitochondrial, ATP synthase, H+ transporting, mitochondrial F1 co, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform1, cardiac muscle, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform2, non-cardiac muscle-like 2, ATP sythase (F1-ATPase) alpha subunit, ATP5A, ATP5A1, ATP5AL2, ATP5AMOM2, ATPM, ATPMATP synthase subunit alpha, mitochondrial, cardiac muscle, COXPD22, EC 3.6.3, EC 3.6.3.14, epididymis secretory sperm binding protein Li 123m, hATP1, HEL-S-123m, MC5DN4, MC5DN4A, MC5DN4B, mitochondrial ATP synthetase, oligomycin-resistant, MOM2, OMR, ORM, ORMATP synthase alpha chain, mitochondrial
Gene Symbol
ATP5F1A
Additional ATP5A Products
Product Documents for ATP5A Antibody (5G11)
Product Specific Notices for ATP5A Antibody (5G11)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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