ATP5H Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47545

Novus Biologicals, part of Bio-Techne
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NBP2-47545
NBP2-47545-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ATP5H Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: ALKVPVPEDKYTAQVDAEEKEDVKSCAEWVSLSKARIVEYEKEMEKMKNLIPFDQMTIEDLNEAFPETKLDKKKYPYWPHQPIENL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%) (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATP5H Antibody

Immunocytochemistry/ Immunofluorescence: ATP5H Antibody [NBP2-47545]

Immunocytochemistry/ Immunofluorescence: ATP5H Antibody [NBP2-47545]

Immunocytochemistry/Immunofluorescence: ATP5H Antibody [NBP2-47545] - Staining of human cell line U-251 MG shows localization to mitochondria.
Immunohistochemistry-Paraffin: ATP5H Antibody [NBP2-47545]

Immunohistochemistry-Paraffin: ATP5H Antibody [NBP2-47545]

Immunohistochemistry-Paraffin: ATP5H Antibody [NBP2-47545] - Staining of human liver.
Immunohistochemistry-Paraffin: ATP5H Antibody [NBP2-47545]

Immunohistochemistry-Paraffin: ATP5H Antibody [NBP2-47545]

Immunohistochemistry-Paraffin: ATP5H Antibody [NBP2-47545] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
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Applications for ATP5H Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATP5H

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, F0, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The F0 seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the F0 complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15. [provided by RefSeq]

Alternate Names

ATP synthase D chain, mitochondrial, ATP synthase subunit d, mitochondrial, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d, ATP synthase, H+ transporting, mitochondrial F1F0, subunit d, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit d, ATP5JD, ATPase subunit d, ATPQ, My032 protein

Gene Symbol

ATP5PD

Additional ATP5H Products

Product Documents for ATP5H Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATP5H Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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