ATP5H Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92443

Novus Biologicals, part of Bio-Techne
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NBP2-92443-0.02ml
NBP2-92443-0.1ml
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ATP5H Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-161 of human ATP5H (NP_006347.1). MAGRKLALKTIDWVAFAEIIPQNQKAIASSLKSWNETLTSRLAALPENPPAIDWAYYKANVAKAGLVDDFEKKFNALKVPVPEDKYTAQVDAEEKEDVKSCAEWVSLSKARIVEYEKEMEKMKNLIPFDQMTIEDLNEAFPETKLDKKKYPYWPHQPIENL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATP5H Antibody - BSA Free

Western Blot: ATP5H AntibodyBSA Free [NBP2-92443]

Western Blot: ATP5H AntibodyBSA Free [NBP2-92443]

Western Blot: ATP5H Antibody [NBP2-92443] - Analysis of extracts of various cell lines, using ATP5H at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
Immunocytochemistry/ Immunofluorescence: ATP5H Antibody - BSA Free [NBP2-92443]

Immunocytochemistry/ Immunofluorescence: ATP5H Antibody - BSA Free [NBP2-92443]

Immunocytochemistry/Immunofluorescence: ATP5H Antibody [NBP2-92443] - Confocal immunofluorescence analysis of U2OS cells using ATP5H Polyclonal Antibody (NBP2-92443) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: ATP5H Antibody - BSA Free [NBP2-92443]

Immunohistochemistry-Paraffin: ATP5H Antibody - BSA Free [NBP2-92443]

Immunohistochemistry-Paraffin: ATP5H Antibody [NBP2-92443] - Immunohistochemistry of paraffin-embedded mouse kidney using ATP5H Rabbit pAb (NBP2-92443) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for ATP5H Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATP5H

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, F0, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The F0 seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the F0 complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15. [provided by RefSeq]

Alternate Names

ATP synthase D chain, mitochondrial, ATP synthase subunit d, mitochondrial, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d, ATP synthase, H+ transporting, mitochondrial F1F0, subunit d, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit d, ATP5JD, ATPase subunit d, ATPQ, My032 protein

Gene Symbol

ATP5PD

Additional ATP5H Products

Product Documents for ATP5H Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ATP5H Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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