ATP5H Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97512

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ATP5H Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human ATP5H fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ATP5H Antibody

Western Blot: ATP5H Antibody [NBP2-97512]

Western Blot: ATP5H Antibody [NBP2-97512]

Western Blot: ATP5H Antibody [NBP2-97512] - Anti-ATP5H rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lane C: PC-3 Whole Cell Lysate. Lane D: U251MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 20 kDa
Immunohistochemistry-Paraffin: ATP5H Antibody [NBP2-97512]

Immunohistochemistry-Paraffin: ATP5H Antibody [NBP2-97512]

Immunohistochemistry-Paraffin: ATP5H Antibody [NBP2-97512] - Immunochemical staining of human ATP5H in human liver with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.
Immunohistochemistry-Paraffin: ATP5H Antibody [NBP2-97512]

Immunohistochemistry-Paraffin: ATP5H Antibody [NBP2-97512]

Immunohistochemistry-Paraffin: ATP5H Antibody [NBP2-97512] - Immunochemical staining of human ATP5H in human kidney with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.
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Applications for ATP5H Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100-1:400

Immunoprecipitation

0.5-2uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATP5H

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, F0, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The F0 seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the F0 complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15. [provided by RefSeq]

Alternate Names

ATP synthase D chain, mitochondrial, ATP synthase subunit d, mitochondrial, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d, ATP synthase, H+ transporting, mitochondrial F1F0, subunit d, ATP synthase, H+ transporting, mitochondrial Fo complex, subunit d, ATP5JD, ATPase subunit d, ATPQ, My032 protein

Gene Symbol

ATP5PD

Additional ATP5H Products

Product Documents for ATP5H Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ATP5H Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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