Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

56.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATP6V1B1 Antibody (OTI1H6) - Azide and BSA Free

Western Blot: ATP6V1B1 Antibody (OTI1H6)Azide and BSA Free [NBP2-70237]

Western Blot: ATP6V1B1 Antibody (OTI1H6) - Azide and BSA Free [NBP2-70237] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V1B1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP6V1B1.

Immunocytochemistry/ Immunofluorescence: ATP6V1B1 Antibody (OTI1H6) - Azide and BSA Free [NBP2-70237]

Immunocytochemistry/Immunofluorescence: ATP6V1B1 Antibody (OTI1H6) - Azide and BSA Free [NBP2-70237] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP6V1B1.

Immunohistochemistry: ATP6V1B1 Antibody (OTI1H6) - Azide and BSA Free [NBP2-70237]

Immunohistochemistry: ATP6V1B1 Antibody (OTI1H6) - Azide and BSA Free [NBP2-70237] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ATP6V1B1 mouse monoclonal antibody.

View 4 more images

Applications for ATP6V1B1 Antibody (OTI1H6) - Azide and BSA Free

Application

Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATP6V1B1

ATP6V1B1 encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain B subunit isoforms and is found in the kidney. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness. [provided by RefSeq]

Alternate Names

ATP6B158kD subunit, ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1, Endomembrane proton pump 58 kDa subunit, H+-ATPase beta 1 subunit, vacuolar proton pump 3, Vacuolar proton pump subunit B 1, vacuolar proton pump, subunit 3, VATBMGC32642, V-ATPase B1 subunit, V-ATPase subunit B 1, Vma2, V-type proton ATPase subunit B, kidney isoform

Gene Symbol

ATP6V1B1

Additional ATP6V1B1 Products

Product Documents for ATP6V1B1 Antibody (OTI1H6) - Azide and BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for ATP6V1B1 Antibody (OTI1H6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Calculators
Flow Cytometry

ATP6V1B1 Antibody (OTI1H6) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70237

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Format

Concentration

View all ATP6V1B1 Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Clonality

Host

Isotype

Theoretical MW

Scientific Data Images for ATP6V1B1 Antibody (OTI1H6) - Azide and BSA Free

Western Blot: ATP6V1B1 Antibody (OTI1H6)Azide and BSA Free [NBP2-70237]

Immunocytochemistry/ Immunofluorescence: ATP6V1B1 Antibody (OTI1H6) - Azide and BSA Free [NBP2-70237]

Immunohistochemistry: ATP6V1B1 Antibody (OTI1H6) - Azide and BSA Free [NBP2-70237]

Flow Cytometry: ATP6V1B1 Antibody (OTI1H6) - Azide and BSA Free [NBP2-70237]

Immunohistochemistry: ATP6V1B1 Antibody (OTI1H6) - Azide and BSA Free [NBP2-70237]

Flow Cytometry: ATP6V1B1 Antibody (OTI1H6) - Azide and BSA Free [NBP2-70237]

Flow Cytometry: ATP6V1B1 Antibody (OTI1H6) - Azide and BSA Free [NBP2-70237]

Applications for ATP6V1B1 Antibody (OTI1H6) - Azide and BSA Free

Flow Cytometry

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Western Blot

Formulation, Preparation, and Storage

Purification

Reconstitution

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: ATP6V1B1

Alternate Names

Gene Symbol

Additional ATP6V1B1 Products

Product Documents for ATP6V1B1 Antibody (OTI1H6) - Azide and BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for ATP6V1B1 Antibody (OTI1H6) - Azide and BSA Free