ATP6V1E1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06402
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human ATP6V1E1 fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for ATP6V1E1 Antibody
Western Blot: ATP6V1E1 Antibody [NBP3-06402]
Western Blot: ATP6V1E1 Antibody [NBP3-06402] - ATP6V1E1 was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate. 4 uL anti-ATP6V1E1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-ATP6V1E1 rabbit polyclonal antibody,at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size :34 kDa.Immunohistochemistry-Paraffin: ATP6V1E1 Antibody [NBP3-06402]
Immunohistochemistry-Paraffin: ATP6V1E1 Antibody [NBP3-06402] - Immunochemical staining of human ATP6V1E1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.Western Blot: ATP6V1E1 Antibody [NBP3-06402]
Western Blot: ATP6V1E1 Antibody [NBP3-06402] - Anti-ATP6V1E1 rabbit polyclonal antibody at 1:500 dilution. Lane A: A-673 Whole Cell Lysate Lane B: HEK-293 Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lane D: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size:34 kDa.Applications for ATP6V1E1 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:50-1:200
Immunoprecipitation
1-5uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ATP6V1E1
Alternate Names
ATP6E2ATP6V1E, ATP6E31kDa subunit, ATPase, H+ transporting, lysosomal (vacuolar proton pump) 31kD, ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E isoform 1, ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1, H+-transporting ATP synthase chain E, vacuolar, p31, Vacuolar proton pump subunit E 1, V-ATPase 31 kDa subunit, V-ATPase subunit E 1, V-ATPase, subunit E, Vma4, V-type proton ATPase subunit E 1
Gene Symbol
ATP6V1E1
Additional ATP6V1E1 Products
Product Specific Notices for ATP6V1E1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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