ATP6V1E1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06402

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ATP6V1E1 Antibodies »

Product Summary for ATP6V1E1 Antibody

Immunogen

Produced in rabbits immunized with E. coli-derived Human ATP6V1E1 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ATP6V1E1 Antibody

Western Blot: ATP6V1E1 Antibody [NBP3-06402]

Western Blot: ATP6V1E1 Antibody [NBP3-06402]

Western Blot: ATP6V1E1 Antibody [NBP3-06402] - ATP6V1E1 was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate. 4 uL anti-ATP6V1E1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-ATP6V1E1 rabbit polyclonal antibody,at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size :34 kDa.
Immunohistochemistry-Paraffin: ATP6V1E1 Antibody [NBP3-06402]

Immunohistochemistry-Paraffin: ATP6V1E1 Antibody [NBP3-06402]

Immunohistochemistry-Paraffin: ATP6V1E1 Antibody [NBP3-06402] - Immunochemical staining of human ATP6V1E1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Western Blot: ATP6V1E1 Antibody [NBP3-06402]

Western Blot: ATP6V1E1 Antibody [NBP3-06402]

Western Blot: ATP6V1E1 Antibody [NBP3-06402] - Anti-ATP6V1E1 rabbit polyclonal antibody at 1:500 dilution. Lane A: A-673 Whole Cell Lysate Lane B: HEK-293 Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lane D: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Observed band size:34 kDa.

Applications for ATP6V1E1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATP6V1E1

ATP6V1E1 encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome. [provided by RefSeq]

Alternate Names

ATP6E2ATP6V1E, ATP6E31kDa subunit, ATPase, H+ transporting, lysosomal (vacuolar proton pump) 31kD, ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E isoform 1, ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1, H+-transporting ATP synthase chain E, vacuolar, p31, Vacuolar proton pump subunit E 1, V-ATPase 31 kDa subunit, V-ATPase subunit E 1, V-ATPase, subunit E, Vma4, V-type proton ATPase subunit E 1

Gene Symbol

ATP6V1E1

Additional ATP6V1E1 Products

Product Documents for ATP6V1E1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ATP6V1E1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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