Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ATP6V1H Antibodies »

Product Summary for ATP6V1H Antibody

Immunogen

The immunogen this antibody was made to, maps to a region between residue 400 to 450 of human ATPase, H+ Transporting, Lysosomal 50/57kDa, V1 Subunit H using the numbering given in entry NP_057025.2 (GeneID 51606).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATP6V1H Antibody

Western Blot: ATP6V1H Antibody [NBP2-32203]

Western Blot: ATP6V1H Antibody [NBP2-32203]

Western Blot: ATP6V1H Antibody [NBP2-32203] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-ATP6V1H antibody NBP2-32203 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: ATP6V1H Antibody [NBP2-32203]

Immunoprecipitation: ATP6V1H Antibody [NBP2-32203]

Immunoprecipitation: ATP6V1H Antibody [NBP2-32203] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ATP6V1H antibody NBP2-32203 used for IP at 6 ug per reaction. ATP6V1H was also immunoprecipitated by rabbit anti-ATP6V1H antibody BL15221. For blotting immunoprecipitated ATP6V1H, NBP2-32203 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for ATP6V1H Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:500 - 1:2500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ATP6V1H

ATP6V1H, also known as V-type proton ATPase subunit H, is a 483 amino acid protein that is a subunit of the V1 complex, which is responsible for acidifying eukaryotic cells in intracellular organelles. Studies on this protein have shown a relationship with the following disorders and diseases, osteopetrosis, carcinoma, cholera, colon carcinoma, arthritis, rheumatoid arthritis, neuronitis, tuberculosis, and immunodeficiency. The ATP6V1H protein has also shown an interaction with ATP6V1D, ATP6V1F, ATP6V1B2, ATP6V1E1, and ETHE1 in the oxidative phosphorylation, metabolic, phagosome, lysosome, insulin receptor recycline, and Iron uptake and transport pathways.

Alternate Names

ATPase, H+ transporting, lysosomal 50/57kD, V1 subunit H, ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H, CGI-11, MSTP042, NBP1, Nef-binding protein 1, Protein VMA13 homolog, SFD, SFDalpha, SFDbeta, vacuolar ATP synthase subunit H, vacuolar ATPase subunit H, vacuolar proton pump H subunit, Vacuolar proton pump subunit H, Vacuolar proton pump subunit SFD, V-ATPase 50/57 kDa subunits, V-ATPase H subunit, V-ATPase subunit H, VMA13, V-type proton ATPase subunit H

Gene Symbol

ATP6V1H

UniProt

NP_057025.2 (Human)

Additional ATP6V1H Products

Product Documents for ATP6V1H Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATP6V1H Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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