ATPB Antibody (OTI4E5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02249

Novus Biologicals, part of Bio-Techne
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NBP2-02249
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Key Product Details

Species Reactivity

Human, Rat, Canine, Monkey, Primate

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI4E5

Concentration

0.9 mg/ml

View all ATPB Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ATP5B (NP_001677) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

56.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATPB Antibody (OTI4E5)

Western Blot: ATPB Antibody (OTI4E5) [NBP2-02249]

Western Blot: ATPB Antibody (OTI4E5) [NBP2-02249]

Western Blot: ATPB Antibody (4E5) [NBP2-02249] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATPB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATPB.
Immunocytochemistry/ Immunofluorescence: ATPB Antibody (OTI4E5) [NBP2-02249]

Immunocytochemistry/ Immunofluorescence: ATPB Antibody (OTI4E5) [NBP2-02249]

Immunocytochemistry/Immunofluorescence: ATPB Antibody (4E5) [NBP2-02249] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ATPB.
Immunohistochemistry-Paraffin: ATPB Antibody (OTI4E5) [NBP2-02249]

Immunohistochemistry-Paraffin: ATPB Antibody (OTI4E5) [NBP2-02249]

Immunohistochemistry-Paraffin: ATPB Antibody (4E5) [NBP2-02249] - Staining of paraffin-embedded pancreas tissue using anti-ATPB mouse monoclonal antibody.
View 12 more images

Applications for ATPB Antibody (OTI4E5)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.9 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATPB

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis,utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.

Alternate Names

ATP synthase subunit beta, mitochondrial, ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide, ATPMB, ATPSBmitochondrial ATP synthase beta subunit, EC 3.6.3, EC 3.6.3.14, MGC5231, mitochondrial ATP synthetase, beta subunit

Entrez Gene IDs

670 (Human)

Gene Symbol

ATP5F1B

Additional ATPB Products

Product Documents for ATPB Antibody (OTI4E5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ATPB Antibody (OTI4E5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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