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ATPG Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15525

Novus Biologicals, part of Bio-Techne
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NBP2-15525

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Cited:

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATPG. The exact sequence is proprietary.

Reactivity Notes

Bovine (86%).

Localization

Mitochondrion; Mitochondrion inner membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATPG Antibody

Western Blot: ATPG Antibody [NBP2-15525]

Western Blot: ATPG Antibody [NBP2-15525]

Western Blot: ATPG Antibody [NBP2-15525] - A: HeLa 12% SDS PAGE ATP synthase gamma(mitochondrial) antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: ATPG Antibody [NBP2-15525]

Immunohistochemistry-Paraffin: ATPG Antibody [NBP2-15525]

Immunohistochemistry-Paraffin: ATPG Antibody [NBP2-15525] - Paraffin-embedded mouse cervix. ATP synthase gamma antibody dilution: 1:500.
Western Blot: ATPG Antibody [NBP2-15525]

Western Blot: ATPG Antibody [NBP2-15525]

Western Blot: ATPG Antibody [NBP2-15525] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.

Applications for ATPG Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

Assay dependent

Western Blot

1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ATPG

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the gamma subunit of the catalytic core. Alternatively spliced transcript variants encoding different isoforms have been identified. This gene also has a pseudogene on chromosome 14. [provided by RefSeq]

Alternate Names

ATP synthase gamma chain, mitochondrial, ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1, ATP5CATP synthase subunit gamma, mitochondrial, ATP5CL1mitochondrial ATP synthase, gamma subunit 1, F-ATPase gamma subunit

Gene Symbol

ATP5F1C

UniProt

Additional ATPG Products

Product Documents for ATPG Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ATPG Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.