ATR Antibody (2B5)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-308

Novus Biologicals, part of Bio-Techne
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NB100-308
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Functional, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, In vitro assay, Knockout Validated, Western Blot

Cited:

Functional Assay, Immunoprecipitation, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2B5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ATR Antibodies »

Product Specifications

Immunogen

Recombinant fragment, corresponding to amino acids 710-1100 of Human ATR.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

317 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATR Antibody (2B5)

Western Blot: ATR Antibody (2B5) [NB100-308]

Western Blot: ATR Antibody (2B5) [NB100-308]

Western Blot: ATR Antibody (2B5) [NB100-308] - Human Raji whole cell extract. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: ATR Antibody (2B5) [NB100-308]

Immunocytochemistry/ Immunofluorescence: ATR Antibody (2B5) [NB100-308]

Immunocytochemistry/Immunofluorescence: ATR Antibody (2B5) [NB100-308] - Analysis of HeLa, using antibody at 1:100 dilution.
Western Blot: ATR Antibody (2B5) [NB100-308]

Western Blot: ATR Antibody (2B5) [NB100-308]

Western Blot: ATR Antibody (2B5) [NB100-308] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [2B5] diluted at 1:500.
View 1 more images

Applications for ATR Antibody (2B5)

Application
Recommended Usage

Western Blot

1:500-1:3000
Application Notes
Functional and IP usages reported in (PMID:18202016). Knockout validation (PMID: 31757956). Use In vitro assay reported in scientific literature (PMID: 18202016). IP, Functional, ICC/IF- Assay dependent.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATR

Ataxia Telangiectasia Mutated (ATM) and Rad3- related (ATR) protein is a member of the phosphatidyl-inositol 3-kinase (PI 3-kinase) like family of protein kinases. ATR has a predicted molecular mass of approximately 301 kDa, and it has been shown to be involved in cellular responses to DNA damage. Specifically, it plays a role in checkpoint activation in response to either the presence of stalled replication forks, or by the presence of lesions caused by UV light. Inactivation of ATR is an embryonic lethal event in mice.

Long Name

Ataxia Telangiectasia and Rad 3-related

Alternate Names

FRP1, MEC1, SCKL1

Entrez Gene IDs

545 (Human)

Gene Symbol

ATR

UniProt

Q13535

Additional ATR Products

Product Documents for ATR Antibody (2B5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATR Antibody (2B5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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