ATR [p Ser428] Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05447

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ATR Antibodies »

Product Summary for ATR [p Ser428] Antibody - Azide and BSA Free

Immunogen

A synthetic phosphorylated peptide around S428 of human ATR (NP_001175.2). GISPK

Modification

p Ser428

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

301 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ATR [p Ser428] Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: ATR [p Ser428] Antibody - Azide and BSA Free [NBP3-05447]

Immunocytochemistry/ Immunofluorescence: ATR [p Ser428] Antibody - Azide and BSA Free [NBP3-05447]

Immunocytochemistry/Immunofluorescence: ATR [p Ser428] Antibody [NBP3-05447] - Immunofluorescence analysis of U2OS cells using ATR antibody (NBP3-05447) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: ATR [p Ser428] Antibody - Azide and BSA Free [NBP3-05447]

Immunohistochemistry-Paraffin: ATR [p Ser428] Antibody - Azide and BSA Free [NBP3-05447]

Immunohistochemistry-Paraffin: ATR [p Ser428] Antibody [NBP3-05447] - Rat testis using ATR Rabbit pAb (NBP3-05447) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: ATR [p Ser428] Antibody - Azide and BSA Free [NBP3-05447]

Immunohistochemistry-Paraffin: ATR [p Ser428] Antibody - Azide and BSA Free [NBP3-05447]

Immunohistochemistry-Paraffin: ATR [p Ser428] Antibody [NBP3-05447] - Human testis using ATR Rabbit pAb (NBP3-05447) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Applications for ATR [p Ser428] Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:100 - 1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATR

Ataxia Telangiectasia Mutated (ATM) and Rad3- related (ATR) protein is a member of the phosphatidyl-inositol 3-kinase (PI 3-kinase) like family of protein kinases. ATR has a predicted molecular mass of approximately 301 kDa, and it has been shown to be involved in cellular responses to DNA damage. Specifically, it plays a role in checkpoint activation in response to either the presence of stalled replication forks, or by the presence of lesions caused by UV light. Inactivation of ATR is an embryonic lethal event in mice.

Long Name

Ataxia Telangiectasia and Rad 3-related

Alternate Names

FRP1, MEC1, SCKL1

Gene Symbol

ATR

Additional ATR Products

Product Documents for ATR [p Ser428] Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATR [p Ser428] Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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