ATRX Antibody (CL0537) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43870

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0537

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all ATRX Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P46100, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ATRX Antibody (CL0537) - Azide and BSA Free

ATRX Antibody (CL0537) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: ATRX Antibody [NBP3-43870]

Immunocytochemistry/ Immunofluorescence: ATRX Antibody [NBP3-43870]

Staining in MCF7 cell line with Anti-ATRX monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
ATRX Antibody (CL0537) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: ATRX Antibody [NBP3-43870]

Immunocytochemistry/ Immunofluorescence: ATRX Antibody [NBP3-43870]

Staining in HeLa cell line with Anti-ATRX monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
ATRX Antibody (CL0537) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: ATRX Antibody [NBP3-43870]

Immunocytochemistry/ Immunofluorescence: ATRX Antibody [NBP3-43870]

Staining in U251 cell line with Anti-ATRX monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
View 6 more images

Applications for ATRX Antibody (CL0537) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATRX

ATRX is encoded by this gene contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. The mutations of this gene are associated with an X-linked mental retardation (XLMR) syndrome most often accompanied by alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported.

Alternate Names

alpha thalassemia/mental retardation syndrome X-linked, alpha thalassemia/mental retardation syndrome X-linked (RAD54 (S. cerevisiae)homolog), alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog, S.cerevisiae), ATP-dependent helicase ATRX, ATR2, DNA dependent ATPase and helicase, EC 3.6.1, EC 3.6.4.12, helicase 2, X-linked, Juberg-Marsidi syndrome, MGC2094, MRXHF1, RAD54 homolog, RAD54L, SFM1, SHS, transcriptional regulator ATRX, XH2RAD54, X-linked helicase II, X-linked nuclear protein, XNPZNF-HX, Zinc finger helicase, Znf-HX

Gene Symbol

ATRX

Additional ATRX Products

Product Documents for ATRX Antibody (CL0537) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ATRX Antibody (CL0537) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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