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Key Product Details

Species Reactivity

Epitope Tag

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Rabbits were immunized with AU5 (TDFYLK) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AU5 Epitope Tag Antibody - BSA Free

Western Blot: AU5 Epitope Tag AntibodyBSA Free [NB600-458]

Western Blot: AU5 Epitope Tag AntibodyBSA Free [NB600-458]

Western Blot: AU5 Epitope Tag Antibody [NB600-458] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000).

Applications for AU5 Epitope Tag Antibody - BSA Free

Application
Recommended Usage

ELISA

1:1000 - 1:30000

Immunocytochemistry/ Immunofluorescence

1:100-1:400

Immunoprecipitation

1 - 4 ug/mg lysate

Western Blot

1:1000 -1:30000
Application Notes
ELISA 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: AU5 Epitope Tag

AU5 is a commonly used epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized by immunochemical methods. The recognized AU5 epitope represents the amino acid sequence TDFYLK.

Alternate Names

AU5 epitope tag, TDFYLK epitope tag, TDFYLK tag

Additional AU5 Epitope Tag Products

Product Documents for AU5 Epitope Tag Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for AU5 Epitope Tag Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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