Aurora A Antibody (1F8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22118

Novus Biologicals, part of Bio-Techne
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NBP2-22118
NBP2-22118SS
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Key Product Details

Species Reactivity

Validated:

Human, Rat, Primate

Cited:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1F8

Format

BSA Free

Concentration

1.0 mg/ml

View all Aurora A Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Aurora A expressed in E. coli.

Marker

Mitosis Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aurora A Antibody (1F8) - BSA Free

Western Blot: Aurora A Antibody (1F8)BSA Free [NBP2-22118]

Western Blot: Aurora A Antibody (1F8)BSA Free [NBP2-22118]

Western Blot: Aurora A Antibody (1F8) [NBP2-22118] - Analysis using Aurora A mouse mAb against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), Hela (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysates.
Immunocytochemistry/ Immunofluorescence: Aurora A Antibody (1F8) - BSA Free [NBP2-22118]

Immunocytochemistry/ Immunofluorescence: Aurora A Antibody (1F8) - BSA Free [NBP2-22118]

Immunocytochemistry/Immunofluorescence: Aurora A Antibody (1F8) [NBP2-22118] - Analysis of Hela cells using Aurora A mouse mAb (green). DRAQ5 fluorescent DNA dye (blue).
Flow Cytometry: Aurora A Antibody (1F8) - BSA Free [NBP2-22118]

Flow Cytometry: Aurora A Antibody (1F8) - BSA Free [NBP2-22118]

Flow Cytometry: Aurora A Antibody (1F8) [NBP2-22118] - Analysis of K562 cells using Aurora A mouse mAb (green) and negative control (purple).
View 3 more images

Applications for Aurora A Antibody (1F8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aurora A

Aurora A plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment.Tissue specificity: Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.

Alternate Names

AIK, ARK1, AURA, AURKA, BTAK, STK15, STK6, STK7

Entrez Gene IDs

6790 (Human)

Gene Symbol

AURKA

UniProt

O14965

Additional Aurora A Products

Product Documents for Aurora A Antibody (1F8) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Aurora A Antibody (1F8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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