aurora A-binding protein Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81152

Novus Biologicals, part of Bio-Techne
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NBP1-81152
NBP1-81152-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Virus

Cited:

Virus

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all aurora A-binding protein Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CGVWLDAAALKRRKVQTHLIKPGTKMLTLLPGERKANIYFTQRRAPSTGIHQRSIASFFTLQPGKTNGSDQTSVSSHTESQIN

Reactivity Notes

Virus reactivity reported in scientific literature (PMID:31964734).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for aurora A-binding protein Antibody

Western Blot: aurora A-binding protein Antibody [NBP1-81152]

Western Blot: aurora A-binding protein Antibody [NBP1-81152]

Western Blot: aurora A-binding protein Antibody [NBP1-81152] - Analysis in control (vector only transfected HEK293T lysate) and AUNIP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: aurora A-binding protein Antibody [NBP1-81152]

Immunocytochemistry/ Immunofluorescence: aurora A-binding protein Antibody [NBP1-81152]

Immunocytochemistry/Immunofluorescence: aurora A-binding protein Antibody [NBP1-81152] - Staining of human cell line U-251 MG shows localization to centrosome. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: aurora A-binding protein Antibody [NBP1-81152]

Immunohistochemistry-Paraffin: aurora A-binding protein Antibody [NBP1-81152]

Immunohistochemistry-Paraffin: aurora A-binding protein Antibody [NBP1-81152] - Staining of human bone marrow shows strong cytoplasmic positivity in megakaryocytes.

Applications for aurora A-binding protein Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Knockdown validation (PMID: 31964734).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: aurora A-binding protein

Alternate Names

AIBP, aurora A-binding protein, chromosome 1 open reading frame 135, FLJ14264, MGC2603

Entrez Gene IDs

79000 (Human)

Gene Symbol

AUNIP

Additional aurora A-binding protein Products

Product Documents for aurora A-binding protein Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for aurora A-binding protein Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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