Aurora B Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-294
![Western Blot: Aurora B AntibodyBSA Free [NB100-294]](https://resources.bio-techne.com/images/products/Aurora-B-Antibody---BSA-Free-Western-Blot-NB100-294-img0002.jpg "Western Blot: Aurora B AntibodyBSA Free [NB100-294]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Microarray, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
12.5 mg/ml
Product Specifications
Immunogen
Synthetic peptide corresponding to amino acids 1-19 of human Aurora B with C-terminal added cysteine conjugated to KLH. Uniprot accession no. Q96GD4
Specificity
Aurora B
Marker
Mitosis Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Aurora B Antibody - BSA Free
Western Blot: Aurora B AntibodyBSA Free [NB100-294]
Western Blot: Aurora B Antibody - BSA Free [NB100-294] - Whole extract of PC-12 cells were separated on SDS-PAGE and probed with Anti-Aurora B antibody produced in Rabbit. The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat.Lanes:1. 1:1000 antibody2. 1:2000 antibody3. Negative Control![Immunocytochemistry/ Immunofluorescence: Aurora B Antibody - BSA Free [NB100-294]](https://resources.bio-techne.com/images/products/Aurora-B-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-294-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Aurora B Antibody - BSA Free [NB100-294]")
Immunocytochemistry/ Immunofluorescence: Aurora B Antibody - BSA Free [NB100-294]
Immunocytochemistry/Immunofluorescence: Aurora B Antibody - BSA Free [NB100-294] - HeLa human cervix epitheloid carcinoma cells were fixed and permeabilized with 4% paraformaldehyde followed by cold methanol. Fixed cells were stained with 1:50 Anti-Aurora B antibody produced in rabbit. The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in goat.Applications for Aurora B Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
50-60 ug
Western Blot
1:1000-1:2000
Application Notes
By Western blot, it detects a band of approximately 41 kDa. Detection of the Aurora B band by immunoblotting is specifically inhibited with the immunizing peptide.
Reviewed Applications
Read 5 reviews rated 4.8 using NB100-294 in the following applications:
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
10mM PBS (pH 7.4)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
12.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Aurora B
Alternate Names
Aik2, AIM-1, AIM1, ARK2, AURKB, IPL1, STK12, STK5
Gene Symbol
AURKB
Additional Aurora B Products
Product Documents for Aurora B Antibody - BSA Free
Product Specific Notices for Aurora B Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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