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Axotrophin Antibody (2B9)

Novus Biologicals, part of Bio-Techne | Catalog # H00064844-M01

Novus Biologicals, part of Bio-Techne
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H00064844-M01

Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2B9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

MARCH7 (NP_073737, 66 a.a. ~ 136 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. WYSESEITQGARSRSQNQQRDHDSKRPKLSCTNCTTSAGRNVGNGLNTLSDSSWRHSQVPRSSSMVLGSFG

Specificity

MARCH7 (2B9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for Axotrophin Antibody (2B9)

Western Blot: Axotrophin Antibody (2B9) [H00064844-M01]

Western Blot: Axotrophin Antibody (2B9) [H00064844-M01]

Western Blot: Axotrophin Antibody (2B9) [H00064844-M01] - MARCH7 monoclonal antibody (M01), clone 2B9 Analysis of MARCH7expression in K-562.
Immunocytochemistry/ Immunofluorescence: Axotrophin Antibody (2B9) [H00064844-M01]

Immunocytochemistry/ Immunofluorescence: Axotrophin Antibody (2B9) [H00064844-M01]

Immunocytochemistry/Immunofluorescence: Axotrophin Antibody (2B9) [H00064844-M01] - Analysis of monoclonal antibody to MARCH7 on HeLa cell. Antibody concentration 10 ug/ml.
ELISA: Axotrophin Antibody (2B9) [H00064844-M01]

ELISA: Axotrophin Antibody (2B9) [H00064844-M01]

ELISA: Axotrophin Antibody (2B9) [H00064844-M01] - Detection limit for recombinant GST tagged MARCH7 is approximately 0.03ng/ml as a capture antibody.

Applications for Axotrophin Antibody (2B9)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Axotrophin

E3 ubiquitin-protein ligase which may specifically enhance the E2 activity of HIP2. E3 ubiquitin ligasesaccept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer theubiquitin to targeted substrates

Alternate Names

AXO, AXOT, Axotrophin, E3 ubiquitin-protein ligase MARCH7, EC 6.3.2, EC 6.3.2.-, MARCH-VIIDKFZp586F1122, membrane-associated ring finger (C3HC4) 7, Membrane-associated RING finger protein 7, Membrane-associated RING-CH protein VII, RING finger protein 177, RNF177axotrophin

Entrez Gene IDs

64844 (Human)

Gene Symbol

MARCH7

Additional Axotrophin Products

Product Documents for Axotrophin Antibody (2B9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Axotrophin Antibody (2B9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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