Axotrophin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-93417
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-NFNQESEGRNT corresponding to internal region according to NP_073737.1.
Epitope
C-NFNQESEGRNT
Reactivity Notes
Expected from sequence similarity: Mouse and Rat
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Axotrophin Antibody
![Immunohistochemistry-Paraffin: Axotrophin Antibody [NB100-93417]](https://resources.bio-techne.com/images/products/Axotrophin-Antibody-Immunohistochemistry-Paraffin-NB100-93417-img0001.jpg "Immunohistochemistry-Paraffin: Axotrophin Antibody [NB100-93417]")
Immunohistochemistry-Paraffin: Axotrophin Antibody [NB100-93417]
Immunohistochemistry-Paraffin: Axotrophin Antibody [NB100-93417] - (3.75ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for Axotrophin Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
3.75ug/ml
Peptide ELISA
Detection limit 1:4000
Western Blot
1ug/ml
Application Notes
Western blot: Preliminary experiments gave an approx 125kDa band in Human Spleen and Tonsil lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 78.1kDa according to NP_073737.1. The 125kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Uterus.
IHC: Paraffin embedded Human Uterus.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Axotrophin
Alternate Names
AXO, AXOT, Axotrophin, E3 ubiquitin-protein ligase MARCH7, EC 6.3.2, EC 6.3.2.-, MARCH-VIIDKFZp586F1122, membrane-associated ring finger (C3HC4) 7, Membrane-associated RING finger protein 7, Membrane-associated RING-CH protein VII, RING finger protein 177, RNF177axotrophin
Gene Symbol
MARCH7
UniProt
Additional Axotrophin Products
Product Documents for Axotrophin Antibody
Product Specific Notices for Axotrophin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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