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AZ1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90053

Novus Biologicals, part of Bio-Techne
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NBP1-90053
NBP1-90053-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DFLMLFEGSPSGKKRPASLSTAPSEKGATWNVLDDQPRGFTLPSNARSSSALDSPAGPRRKECTVALAPNFTANNRSNKGAVGNCVTTM

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AZ1 Antibody

Western Blot: AZ1 Antibody [NBP1-90053]

Western Blot: AZ1 Antibody [NBP1-90053]

Western Blot: AZ1 Antibody [NBP1-90053] - Western blot analysis in human cell line U-2 OS.
Immunocytochemistry/ Immunofluorescence: AZ1 Antibody [NBP1-90053]

Immunocytochemistry/ Immunofluorescence: AZ1 Antibody [NBP1-90053]

Immunocytochemistry/Immunofluorescence: AZ1 Antibody [NBP1-90053] - Staining of human cell line A-431 shows positivity in centrosome. Antibody stainig is shown in green.
Immunohistochemistry-Paraffin: AZ1 Antibody [NBP1-90053]

Immunohistochemistry-Paraffin: AZ1 Antibody [NBP1-90053]

Immunohistochemistry-Paraffin: AZ1 Antibody [NBP1-90053] - Staining of human tonsil shows positivity in lymphoid cells.

Applications for AZ1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AZ1

CEP131/AZ1 was originally identified as a transcript that was increased in cells exposed to the demethylating agent, azacytidine. It was found to localize to the pre-acrosome region of spermatids and therefore thought to play a role in spermatogenesis. CEP131/AZ1 has also been identified as a component of the centrosome.

Alternate Names

5-azacytidine induced 1, 5-azacytidine-induced protein 1, AZ1, centrosomal protein 131 kDa, centrosomal protein of 131 kDa, Cep131, KIAA1118, pre-acrosome localization protein 1, ZA1

Gene Symbol

CEP131

Additional AZ1 Products

Product Documents for AZ1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for AZ1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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