B-Raf Antibody (1H12) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47433

Novus Biologicals, part of Bio-Techne
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NBP1-47433
NBP1-47433-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1H12

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all B-Raf Antibodies »

Product Summary for B-Raf Antibody (1H12) - BSA Free

Immunogen

Purified recombinant fragment of human B-Raf expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for B-Raf Antibody (1H12) - BSA Free

Western Blot: B-Raf Antibody (1H12)BSA Free [NBP1-47433]

Western Blot: B-Raf Antibody (1H12)BSA Free [NBP1-47433]

Western Blot: B-Raf Antibody (1H12) [NBP1-47433] - Analysis using BRAF mouse mAb against Hela (1), HL60 (2), HepG2 (3) and NIH/3T3 (4) cell lysate.
Immunohistochemistry-Paraffin: B-Raf Antibody (1H12) - BSA Free [NBP1-47433]

Immunohistochemistry-Paraffin: B-Raf Antibody (1H12) - BSA Free [NBP1-47433]

Immunohistochemistry-Paraffin: B-Raf Antibody (1H12) [NBP1-47433] - Analysis of human testis tissues using BRAF mouse mAb.
Immunohistochemistry-Paraffin: B-Raf Antibody (1H12) - BSA Free [NBP1-47433]

Immunohistochemistry-Paraffin: B-Raf Antibody (1H12) - BSA Free [NBP1-47433]

Immunohistochemistry-Paraffin: B-Raf Antibody (1H12) [NBP1-47433] - Analysis of MCF-7 (left) and HepG2 (right) cells using BRAF mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye

Applications for B-Raf Antibody (1H12) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is useful in Western Blot, Immunohistochemistry-paraffin, Immunocytochemistry/Immunofluorescence and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: B-Raf

B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.

Long Name

v-Raf Murine Sarcoma Viral Oncogene Homolog B1

Alternate Names

BRaf, BRAF1, RAFB1

Entrez Gene IDs

673 (Human); 109880 (Mouse)

Gene Symbol

BRAF

UniProt

P15056

Additional B-Raf Products

Product Documents for B-Raf Antibody (1H12) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for B-Raf Antibody (1H12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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